European training network for development of personalized anti-infective medical devices combining printing technologies and antimicrobial functionality

This publication describes the miniaturization process of a 96-well plate antibacterial screening platform, into a 384-well plate format. The platform is based on a sequential viability and biomass measurements for the assessment of anti-biofilm activity. Different parameters were evaluated using Staphylococcus aureus and Pseudomonas aeruginosa as the bacterial models. A pilot screening of 100 compounds was performed, followed by the screening of a library of 2000 compounds to identify new repurposed anti-biofilm agents. Our results show that the optimized platform is well-suited for screening purposes, allowing for the rapid screening of a higher number of compounds in a reliable manner.